Giới thiệu sách mới - Hóa học

Giới thiệu sách mới - Hóa học

mặc dù HuyNgyuễn chuyên về sách sinh học, công nghệ sinh học, y dược học, tuy nhiên nhận thấy nhu cầu về sách Hóa học của các anh chị không phải là ít và HuyNguyễn hòan toàn có khả năng đáp ứng nhu cầu này , nay HuyNguyễn mạnh dạn sưu tập và cung cấp sách hóa chuyên ngành tiếng ANH theo yêu cầu của các anh chị.

Xin vui lòng email về phongtailieu@huynguyenltd.com để biết thêm chi tiết, hoặc đến 201 Lương Nhữ Học Quận 5, Tp HCM để được hướng dẫn thêm.

Xin giới thiệu 3 trong số những cuốn sách chuyên đề Hóa mà HuyNguyễn vừa cập nhật ngày 14-04-2004. Hiện nay số đầu sách Hóa học tăng lên mỗi ngày, chúng tôi sẽ lần lượt giới thiệu để các anh chi được rõ




Multidimensional Chromatography
E***ed by Luigi Mondello, Alastair C. Lewis and Keith D. Bartle
Copyright © 2002 John Wiley & Sons Ltd
ISBNs: 0-471-98869-3 (Hardback); 0-470-84577-5 (Electronic)


CONTENTS
List of Contributors xi
Preface xiii
PART 1: GENERAL 1
1 Introduction 3
K.D. Bartle
1.1 Preamble 3
1.2 Packed Capillary Column and Unified Chromatography 4
1.3 Resolving Power of Chromatographic Systems 6
1.4 Two-Dimensional Separations 9
1.5 The Origins of Multidimensional Chromatography 12
Acknowledgements 13
References 13
2 Coupled High Performance Liquid Chromatography with High
Resolution Gas Chromatography 17
L. Mondello
2.1 Introduction 17
2.2 Transfer Techniques 18
2.3 Vaporization with Hot Injectors 25
2.4 Transfer of Water-Containing Solvent Mixtures 28
2.5 Indirect Introduction of Water 31
2.6 Conclusions 38
References 42
3 Multidimensional High Resolution Gas Chromatography 47
A. C. Lewis
3.1 Introduction 47
3.2 Practical Two-Dimensional Gas Chromatography 48
3.3 Practical Examples of Two-Dimensional Chromatography 57
3.4 Conclusions 72
References 72
4 Orthogonal GC?"GC 77
P. J. Marriott
4.1 Introduction to Multidimensional Gas Chromatography 77
4.2 Introduction to GC  GC Separation 80
4.3 Introduction to Modulation Technology 82
4.4 Orthogonality of Analysis 94
4.5 Quantitative Aspects 101
4.6 Future Opportunities and Challenges of GC  GC Technology 104
Acknowledgements 106
References 106
5 Coupled-Column Liquid Chromatography 109
Claudio Corradini
5.1 Introduction 109
5.2 Theoretical Aspects 111
5.3 LC?"LC Techniques 116
5.4 Conclusions 129
References 130
6 Supercritical Fluid Techniques Coupled with Chromatographic
Techniques 135
F. M. Lanças
6.1 Introduction 135
6.2 On-Line Coupling of SFE with Chromatographic Techniques 138
6.3 SFE?"GC 138
6.4 SPE?"SFE?"GC 139
6.5 SFE?"SFC 141
6.6 SFE?"LC 141
6.7 On-Line Coupling of Supercritical Fluid Extraction with
Capillary Electrodriven Separation Techniques (SFE?"CESTs) 143
6.8 From Multidimensional to Unified Chromatography
Passing through Supercritical Fluids 147
References 147
7 Unified Chromatography: Concepts and Considerations for
Multidimensional Chromatography 151
T.L. Chester
7.1 Introduction 151
7.2 The Phase Diagram View of Chromatography 153
7.3 Instrumentation 159
7.4 Advantages of and Challenges for Unified Chromatography
Techniques in Multidimensional Systems 162
7.5 Column Efficiency and Plate Heights in
Unified Chromatography 164
References 167
8 Multidimensional Planar Chromatography 171
Sz. Nyiredy
8.1 Introduction 171
8.2 Two-Dimensional or Multidimensional
Planar Chromatography? 172
vi Contents
8.3 Two-Dimensional Development on Single Layers 173
8.4 Methods for the Selection of Appropriate Mobile Phases 175
8.5 Two-Dimensional Development on Bilayers 176
8.6 Multiple Development in One, Two or Three Dimensions 177
8.7 Multiple Development in One Direction 178
8.8 Multiple Development in Two Dimensions 182
8.9 Multiple Development in Three Dimensions 184
8.10 Coupled Layers with Stationary Phases of Decreasing Polarity 186
8.11 Grafted Planar Chromatography 186
8.12 Serially Connected Multilayer Forced-Flow Planar
Chromatography 188
8.13 Combination of Multidimensional Planar
Chromatographic Methods 191
8.14 Coupling of Planar Chromatography with other
Chromatographic Techniques 193
8.15 Further Considerations 193
References 194
9 Multidimensional Electrodriven Separations 197
Martha M. Degen and Vincent T. Remcho
9.1 Introduction 197
9.2 Electrophoretic Separations 197
9.3 Comprehensive Separations 199
9.4 Planar Two-Dimensional Separations 200
9.5 Chromatography and Electrophoresis Combined in
Non-Comprehensive Manners 201
9.6 Comprehensive Two-Dimensional Separations with
an Electrodriven Component 203
9.7 Microcolumn Reverse Phase High Performance Liquid
Chromatography?"Capillary Zone Electrophoresis 204
9.8 Microcolumn Size Exclusion
Chromatography?"Capillary Zone Electrophoresis 206
9.9 Packed Capillary Reverse Phase High Performance
Liquid Chromatography?"Capillary Zone Electrophoresis 207
9.10 Packed Capillary Reverse Phase High Performance Liquid
Chromatography?"Fast Capillary Zone Electrophoresis 208
9.11 Three-Dimensional Size Exclusion Chromatography ?"
Reverse Phase Liquid Chromatography?"
Capillary Zone Electrophoresis 209
9.12 Transparent Flow Grating Interface with Packed Capillary
High Performance Liquid Chromatography?"Capillary
Zone Electrophoresis 210
9.13 Online Reverse Phase High Performance Liquid
Chromatography?" Capillary Zone Electrophoresis?"Mass
Spectrometry 211
Contents vii
9.14 The Future of Multidimensional Electrokinetic Separations 212
9.15 Conclusions 213
References 213
PART 2: APPLICATIONS 215
10 Multidimensional Chromatography: Foods, Flavours and
Fragrances Applications 217
G. Dugo, P. Dugo and L. Mondello
10.1 Introduction 217
10.2 Multidimensional Gas Chromatography (GC?"GC or MDGC) 218
10.3 Multidimensional High Performance Liquid
Chromatography 231
10.4 Multidimensional Chromatography using On-Line Coupled
High Performance Liquid Chromatography and Capillary
(High Resolution) Gas Chromatography (HPLC?"HRGC) 235
10.5 Multidimensional Chromatographic Methods which
Involve the Use of Supercritical Fluids 241
10.6 Multidimensional Planar Chromatography 242
References 245
11 Multidimensional Chromatography: Biomedical and
Pharmaceutical Applications 251
G. W. Somsen and G. J. de Jong
11.1 Introduction 251
11.2 Liquid Chromatography?"Liquid Chromatography 252
11.3 Solid-Phase Extraction?"Liquid Chromatography 265
11.4 Liquid Chromatography?"Gas Chromatography 273
11.5 Solid-Phase Extraction?"Gas Chromatography 278
11.6 Solid-Phase Microextractions Coupled with Gas
or Liquid Chromatography 280
11.7 Supercritical Fluid Extraction Coupled with Supercritical
Fluid Chromatography 284
11.8 Coupled Systems Involving Capillary Electrophoresis 285
11.9 Conclusions 290
References 291
12 Industrial and Polymer Applications 303
Y. V. Kazakevich and R. LoBrutto
12.1 Introduction 303
12.2 General 304
12.3 LC?"GC 304
viii Contents
12.4 LC?"GC Applications 305
12.5 SEC?"GC Applications 306
12.6 SEC?"Reversed Phase LC Applications 315
12.7 GC?"GC Applications 317
12.8 SFC?"GC and Normal Phase-LC?"SFC Applications 324
12.9 Normal Phase-LC?"SFC Applications 324
12.10 SFC?"GC Applications 325
12.11 SFC?"SFC Applications 328
12.12 Conclusions 330
References 331
13 Multidimensional Chromatography in Environmental Analysis 335
R. M. Marcé
13.1 Introduction 335
13.2 Multidimensional Gas Chromatography 336
13.3 Multidimensional Liquid Chromatography 341
13.4 Liquid Chromatography?"Gas Chromatography 358
13.5 Conclusions and Trends 370
Acknowledgements 370
References 370
14 Multidimensional Chromatographic Applications in
the Oil Industry 379
J. Beens
14.1 Introduction 379
14.2 Gases 381
14.3 Gasolines and Naphthas 387
14.4 Middle Distillates 392
14.5 Residue-Containing Products 401
Acknowledgements 403
References 403
15 Multidimensional Chromatography: Forensic and
Toxicological Applications 407
15.1 Introduction 407
15.2 Liquid Chromatography?"Gas Chromatography 408
15.3 Liquid Chromatography?"Liquid Chromatography 410
15.4 Gas Chromatography?"Gas Chromatography 414
15.5 On-Line Sample Preparation 427
15.6 Conclusions 429
Acknowledgements 429
References 429
Index 433



=============================================




The Analysis of Controlled Substances.
Michael D. Cole
Copyright ? 2003 John Wiley & Sons, Ltd.
ISBNs: 0-471-49252-3 (HB); 0-471-49253-1 (PB)


Contents
Series Preface xi
Preface xiii
Acronyms, Abbreviations and Symbols xv
About the Author xvii
1 Introduction to Drug Trends, Control, Legislation and Analysis 1
1.1 Introductory Remarks 1
1.2 International Legislation 2
1.3 Controlled Substances in the United Kingdom 3
1.3.1 Background to the Misuse of Drugs Act, 1971 3
1.3.2 The Provisions of the Misuse of Drugs Act, 1971 3
1.4 Controlled Substances in the United States 5
1.5 Controlled Substances in Australia 5
1.6 The Drug Chemist and Drug Analysis 5
1.7 Quality Assurance in the Drugs Laboratory 9
1.8 Presentation of Evidence in Court 10
References 11
2 Amphetamine and Related Compounds 13
2.1 Introduction 13
2.2 Qualitative Identification of Amphetamines 15
2.2.1 Sampling and Physical Description of Amphetamines 15
2.2.2 Presumptive Testing of Amphetamines 18
2.2.3 Thin Layer Chromatography of Amphetamines 19
2.2.4 Definitive Identification of Amphetamines 20
viii Analysis of Controlled Substances
2.3 Quantification of Amphetamines 25
2.4 Comparison and Profiling of Amphetamine Samples 31
2.4.1 The Leuckart Synthesis of Amphetamine 32
2.4.2 The Reductive Amination of Benzyl Methyl Ketone 32
2.4.3 The Nitrostyrene Synthesis 33
2.4.4 Impurity Extraction and Sample Comparison 34
References 36
3 The Analysis of LSD 37
3.1 Introduction 37
3.2 Qualitative Identification of LSD 38
3.2.1 Sampling and Physical Description of LSD Blotter Acid 39
3.2.2 Extraction of LSD Prior to Analysis 41
3.2.3 Presumptive Testing for LSD 42
3.2.4 Thin Layer Chromatography of Samples Containing LSD 43
3.2.5 Confirmatory Tests for the Presence of LSD 43
References 47
4 Cannabis sativa and Products 49
4.1 Introduction 49
4.2 Origins, Sources and Manufacture of Cannabis 51
4.3 Analytical Sequence, Bulk and Trace Sampling Procedures 53
4.4 Qualitative Identification of Cannabis 54
4.4.1 Identification of Herbal Material 55
4.4.2 Identification of Other Materials 57
4.4.3 Comparison of Cannabis Samples 65
References 72
5 Diamorphine and Heroin 73
5.1 Introduction 73
5.2 Origins, Sources and Manufacture of Diamorphine 74
5.3 Appearance of Heroin and Associated Paraphernalia 77
5.4 Bulk and Trace Sampling Procedures 78
5.5 Identification, Quantification and Comparison of Heroin Samples 79
5.5.1 Presumptive Tests for Heroin 80
5.5.2 Thin Layer Chromatography of Heroin Samples 81
5.5.3 Gas Chromatographic?"Mass Spectroscopic
Identification of Heroin 83
5.5.4 Quantification of Heroin Samples 87
5.5.5 Comparison of Heroin Samples 92
References 95
Contents ix
6 Cocaine 97
6.1 Introduction 97
6.2 Origins, Sources and Manufacture of Cocaine 99
6.2.1 Extraction and Preparation of Coca Paste 99
6.2.2 Synthesis of Pure Cocaine 100
6.3 Qualitative Identification of Cocaine 100
6.3.1 Presumptive Tests for Cocaine 100
6.3.2 Thin Layer Chromatography 102
6.3.3 Definitive Identification of Cocaine 103
6.4 Quantification of Cocaine 107
6.4.1 Quantification of Cocaine by GC?"MS 107
6.4.2 Quantification of Cocaine by UV Spectroscopy 108
6.5 Comparison of Cocaine Samples 109
References 110
7 Products from Catha edulis and Lophophora williamsii 113
7.1 Introduction 113
7.2 Products of Catha edulis 113
7.2.1 Identification, Quantification and Comparison
of Khat Samples 115
7.2.2 Comparison of Khat Samples 119
7.3 Products of Lophophora williamsii 119
7.3.1 Physical Description and Sampling of Materials 120
7.3.2 Presumptive Tests for Mescaline 121
7.3.3 TLC Analysis of Mescaline 121
7.3.4 HPLC Analysis of Mescaline 122
7.3.5 GC?"MS Analysis of Mescaline 124
7.3.6 Comparison of Peyote Samples 124
References 125
8 The Analysis of Psilocybin and Psilocin from Fungi 127
8.1 Introduction 127
8.2 Identification of Psilocybin- and Psilocin-Containing Mushrooms 129
8.2.1 Identification of Fungal Species from Morphological
Characteristics 129
8.2.2 Identification of Psilocin and Psilocybin Using
Chemical Analysis 130
8.2.3 Quantification of Psilocin and Psilocybin by HPLC 135
8.3 The Identification of Psilocybin- and Psilocin-Containing
Fungi Using DNA Profiling 136
References 137
x Analysis of Controlled Substances
9 The Analysis of Controlled Pharmaceutical Drugs ?" Barbiturates
and Benzodiazepines 139
9.1 Introduction 139
9.2 Analysis of Barbiturates and Benzodiazepines 141
9.2.1 Extraction of Barbiturates and Benzodiazepines
from Dose Forms 142
9.2.2 Presumptive Tests for Barbiturates and Benzodiazepines 142
9.2.3 TLC of Barbiturates and Benzodiazepines 143
9.2.4 Confirmatory Analysis of Barbiturates
and Benzodiazepines 146
9.2.5 Quantification of Barbiturates and Benzodiazepines 149
References 151
10 Current Status, Summary and Conclusions 153
10.1 Current Status of Drug Analysis 153
10.2 Conclusions 155
Appendices 157
1 Presumptive (Colour) Tests 157
2 Less-Common Controlled Substances 159
Responses to Self-Assessment Questions 161
Bibliography 175
Glossary of Terms 179
SI Units and Physical Constants 183
Periodic Table 187
Index 189

Sample Preparation Techniques in Analytical Chemistry
Published Online: 28 Oct 2003
E***or(s): Somenath Mitra
Print ISBN: 0471328456 Online ISBN: 0471457817
Copyright â 2003 John Wiley & Sons, Inc.
CONTENTS
CONTRIBUTORS xvii
PREFACE xix
CHAPTER 1 SAMPLE PREPARATION: AN
ANALYTICAL PERSPECTIVE 1
Somenath Mitra and Roman Brukh
1.1. The Measurement Process 1
1.1.1. Qualitative and Quantitative
Analysis 3
1.1.2. Methods of Quantitation 4
1.2. Errors in Quantitative Analysis: Accuracy
and Precision 6
1.2.1. Accuracy 6
1.2.2. Precision 6
1.2.3. Statistical Aspects of Sample
Preparation 10
1.3. Method Performance and Method
Validation 12
1.3.1. Sensitivity 13
1.3.2. Detection Limit 14
1.3.3. Range of Quantitation 15
1.3.4. Other Important Parameters 15
1.3.5. Method Validation 16
1.4. Preservation of Samples 17
1.4.1. Volatilization 19
1.4.2. Choice of Proper Containers 19
1.4.3. Absorption of Gases from the
Atmosphere 20
1.4.4. Chemical Changes 20
1.4.5. Preservation of Unstable Solids 20
vii
1.5. Postextraction Procedures 21
1.5.1. Concentration of Sample Extracts 21
1.5.2. Sample Cleanup 22
1.6. Quality Assurance and Quality Control
during Sample Preparation 25
1.6.1. Determination of Accuracy and
Precision 28
1.6.2. Statistical Control 29
1.6.3. Matrix Control 31
1.6.4. Contamination Control 32
References 35
SECTION A EXTRACTION AND ENRICHMENT IN
SAMPLE PREPARATION
CHAPTER 2 PRINCIPLES OF EXTRACTION AND THE
EXTRACTION OF SEMIVOLATILE
ORGANICS FROM LIQUIDS 37
Martha J. M. Wells
2.1. Principles of Extraction 37
2.1.1. Volatilization 38
2.1.2. Hydrophobicity 43
2.1.3. Acidõ?"Base Equilibria 50
2.1.4. Distribution of Hydrophobic
Ionogenic Organic Compounds 57
2.2. Liquidõ?"Liquid Extraction 57
2.2.1. Recovery 60
2.2.2. Methodology 66
2.2.3. Procedures 68
2.2.4. Recent Advances in Techniques 72
2.3. Liquidõ?"Solid Extraction 74
2.3.1. Sorption 75
2.4. Solid-Phase Extraction 78
2.4.1. Sorbents in SPE 81
2.4.2. Sorbent Selection 96
2.4.3. Recovery 99
2.4.4. Methodology 108
viii contents
2.4.5. Procedures 111
2.4.6. Recent Advances in SPE 113
2.5. Solid-Phase Microextraction 113
2.5.1. Sorbents 116
2.5.2. Sorbent Selection 118
2.5.3. Methodology 119
2.5.4. Recent Advances in Techniques 124
2.6. Stir Bar Sorptive Extraction 125
2.6.1. Sorbent and Analyte Recovery 125
2.6.2. Methodology 127
2.6.3. Recent Advances in Techniques 129
2.7. Method Comparison 130
References 131
CHAPTER 3 EXTRACTION OF SEMIVOLATILE
ORGANIC COMPOUNDS FROM SOLID
MATRICES 139
Dawen Kou and Somenath Mitra
3.1. Introduction 139
3.1.1. Extraction Mechanism 140
3.1.2. Preextraction Procedures 141
3.1.3. Postextraction Procedures 141
3.2. Soxhlet and Automated Soxhlet 142
3.2.1. Soxhlet Extraction 142
3.2.2. Automated Soxhlet Extraction 143
3.2.3. Comparison between Soxtec and
Soxhlet 145
3.3. Ultrasonic Extraction 145
3.3.1. Selected Applications and
Comparison with Soxhlet 147
3.4. Supercritical Fluid Extraction 148
3.4.1. Theoretical Considerations 148
3.4.2. Instrumentation 152
3.4.3. Operational Procedures 153
3.4.4. Advantages/Disadvantages and
Applications of SFE 154
3.5. Accelerated Solvent Extraction 155
ix contents
3.5.1. Theoretical Considerations 155
3.5.2. Instrumentation 156
3.5.3. Operational Procedures 158
3.5.4. Process Parameters 159
3.5.5. Advantages and Applications of
ASE 161
3.6. Microwave-Assisted Extraction 163
3.6.1. Theoretical Considerations 163
3.6.2. Instrumentation 164
3.6.3. Procedures and Advantages/
Disadvantages 170
3.6.4. Process Parameters 170
3.6.5. Applications of MAE 173
3.7. Comparison of the Various Extraction
Techniques 173
References 178
CHAPTER 4 EXTRACTION OF VOLATILE ORGANIC
COMPOUNDS FROM SOLIDS AND
LIQUIDS 183
Gregory C. Slack, Nicholas H. Snow, and Dawen Kou
4.1. Volatile Organics and Their Analysis 183
4.2. Static Headspace Extraction 184
4.2.1. Sample Preparation for Static
Headspace Extraction 186
4.2.2. Optimizing Static Headspace
Extraction Eõ?ciency and
Quantitation 187
4.2.3. Quantitative Techniques in Static
Headspace Extraction 190
4.3. Dynamic Headspace Extraction or Purge
and Trap 194
4.3.1. Instrumentation 194
4.3.2. Operational Procedures in Purge
and Trap 199
4.3.3. Interfacing Purge and Trap with
GC 199
4.4. Solid-Phase Microextraction 200
x contents
4.4.1. SPME Method Development for
Volatile Organics 201
4.4.2. Choosing an SPME Fiber Coating 204
4.4.3. Optimizing Extraction Con***ions 206
4.4.4. Optimizing SPMEõ?"GC Injection 207
4.5. Liquidõ?"Liquid Extraction with Large-
Volume Injection 208
4.5.1. Large-Volume GC Injection
Techniques 208
4.5.2. Liquidõ?"Liquid Extraction for
Large-Volume Injection 211
4.6. Membrane Extraction 212
4.6.1. Membranes and Membrane
Modules 215
4.6.2. Membrane Introduction Mass
Spectrometry 217
4.6.3. Membrane Extraction with Gas
Chromatography 218
4.6.4. Optimization of Membrane
Extraction 222
4.7. Conclusions 223
References 223
CHAPTER 5 PREPARATION OF SAMPLES FOR
METALS ANALYSIS 227
Barbara B. Kebbekus
5.1. Introduction 227
5.2. Wet Digestion Methods 230
5.2.1. Acid Digestionõ?"Wet Ashing 231
5.2.2. Microwave Digestion 234
5.2.3. Comparison of Digestion Methods 235
5.2.4. Pressure Ashing 237
5.2.5. Wet Ashing for Soil Samples 237
5.3. Dry Ashing 240
5.3.1. Organic Extraction of Metals 241
5.3.2. Extraction with Supercritical Fluids 244
5.3.3. Ultrasonic Sample Preparation 245
xi contents
5.4. Solid-Phase Extraction for Preconcentration 245
5.5. Sample Preparation for Water Samples 248
5.6. Precipitation Methods 251
5.7. Preparation of Sample Slurries for Direct
AAS Analysis 251
5.8. Hydride Generation Methods 252
5.9. Colorimetric Methods 254
5.10. Metal Speciation 255
5.10.1. Types of Speciation 257
5.10.2. Speciation for Soils and Sediments 258
5.10.3. Sequential Schemes for Metals in
Soil or Sediment 259
5.10.4. Speciation for Metals in Plant
Materials 260
5.10.5. Speciation of Specific Elements 262
5.11. Contamination during Metal Analysis 263
5.12. Safe Handling of Acids 264
References 264
SECTION B SAMPLE PREPARATION FOR NUCLEIC
ACID ANALYSIS
CHAPTER 6 SAMPLE PREPARATION IN DNA
ANALYSIS 271
Satish Parimoo and Bhama Parimoo
6.1. DNA and Its Structure 271
6.1.1. Physical and Chemical Properties of
DNA 274
6.1.2. Isolation of DNA 276
6.2. Isolation of DNA from Bacteria 278
6.2.1. Phenol Extraction and Precipitation
of DNA 278
6.2.2. Removal of Contaminants from
DNA 282
6.3. Isolation of Plasmid DNA 283
6.3.1. Plasmid DNA Preparation 284
6.3.2. Purification of Plasmid DNA 285
6.4. Genomic DNA Isolation from Yeast 287
xii contents
6.5. DNA from Mammalian Tissues 288
6.5.1. Blood 288
6.5.2. Tissues and Tissue Culture Cells 289
6.6. DNA from Plant Tissue 290
6.7. Isolation of Very High Molecular Weight
DNA 290
6.8. DNA Amplification by Polymerase Chain
Reaction 291
6.8.1. Starting a PCR Reaction 291
6.8.2. Isolation of DNA from Small Real-
World Samples for PCR 294
6.9. Assessment of Quality and Quantitation of
DNA 296
6.9.1. Precautions for Preparing DNA 296
6.9.2. Assessment of Concentration and
Quality 296
6.9.3. Storage of DNA 299
References 299
CHAPTER 7 SAMPLE PREPARATION IN RNA
ANALYSIS 301
Bhama Parimoo and Satish Parimoo
7.1. RNA: Structure and Properties 301
7.1.1. Types and Location of Various
RNAs 303
7.2. RNA Isolation: Basic Considerations 306
7.2.1. Methods of Extraction and
Isolation of RNA 307
7.3. Phenol Extraction and RNA Recovery:
Basic Principles 309
7.3.1. Examples of RNA Isolation Using
Phenol Extraction 310
7.4. Guanidinium Salt Method 313
7.4.1. Examples of RNA Isolation Using
Guanidinium Salts 313
7.5. Isolation of RNA from Nuclear and
Cytoplasmic Cellular Fractions 317
xiii contents
7.6. Removal of DNA Contamination from
RNA 317
7.7. Fractionation of RNA Using
Chromatography Methods 318
7.7.1. Fractionation of Small RNA by
HPLC 318
7.7.2. mRNA Isolation by Aõ?nity
Chromatography 319
7.8. Isolation of RNA from Small Numbers of
Cells 323
7.9. In Vitro Synthesis of RNA 324
7.10. Assessment of Quality and Quantitation of
RNA 326
7.11. Storage of RNA 328
References 329
CHAPTER 8 TECHNIQUES FOR THE EXTRACTION,
ISOLATION, AND PURIFICATION OF
NUCLEIC ACIDS 331
Mahesh Karwa and Somenath Mitra
8.1. Introduction 331
8.2. Methods of Cell Lysis 333
8.2.1. Mechanical Methods of Cell Lysis 335
8.2.2. Nonmechanical Methods of Cell
Lysis 339
8.3. Isolation of Nucleic Acids 342
8.3.1. Solvent Extraction and
Precipitation 344
8.3.2. Membrane Filtration 345
8.4. Chromatographic Methods for the
Purification of Nucleic Acids 346
8.4.1. Size-Exclusion Chromatography 347
8.4.2. Anion-Exchange Chromatography 348
8.4.3. Solid-Phase Extraction 351
8.4.4. Aõ?nity Purification 352
8.5. Automated High-Throughput DNA
Purification Systems 355
8.6. Electrophoretic Separation of Nucleic Acids 360
xiv contents
8.6.1. Gel Electrophoresis for Nucleic
Acids Purification 360
8.6.2. Techniques for the Isolation of
DNA from Gels 362
8.7. Capillary Electrophoresis for Sequencing
and Sizing 364
8.8. Microfabricated Devices for Nucleic Acids
Analysis 366
8.8.1. Sample Preparation on Microchips 370
References 373
SECTION C SAMPLE PREPARATION IN MICROSCOPY
AND SPECTROSCOPY
CHAPTER 9 SAMPLE PREPARATION FOR
MICROSCOPIC AND SPECTROSCOPIC
CHARACTERIZATION OF SOLID
SURFACES AND FILMS 377
Sharmila M. Mukhopadhyay
9.1. Introduction 377
9.1.1. Microscopy of Solids 378
9.1.2. Spectroscopic Techniques for Solids 381
9.2. Sample Preparation for Microscopic
Evaluation 382
9.2.1. Sectioning and Polishing 382
9.2.2. Chemical and Thermal Etching 385
9.2.3. Sample Coating Techniques 387
9.3. Specimen Thinning for TEM Analysis 389
9.3.1. Ion Milling 391
9.3.2. Reactive Ion Techniques 393
9.3.3. Chemical Polishing and
Electropolishing 394
9.3.4. Tripod Polishing 396
9.3.5. Ultramicrotomy 398
9.3.6. Special Techniques and Variations 399
9.4. Summary: Sample Preparation for
Microscopy 400
xv contents
9.5. Sample Preparation for Surface
Spectroscopy 402
9.5.1. Ion Bombardment 407
9.5.2. Sample Heating 408
9.5.3. In Situ Abrasion and Scraping 408
9.5.4. In Situ Cleavage or Fracture Stage 408
9.5.5. Sample Preparation/Treatment
Options for In Situ Reaction
Studies 409
9.6. Summary: Sample Preparation for Surface
Spectroscopy 409
References 410
CHAPTER 10 SURFACE ENHANCEMENT BY SAMPLE
AND SUBSTRATE PREPARATION
TECHNIQUES IN RAMAN AND INFRARED
SPECTROSCOPY 413
Zafar Iqbal
10.1. Introduction 413
10.1.1. Raman EÔect 413
10.1.2. Fundamentals of Surface-Enhanced
Raman Spectroscopy 415
10.1.3. Attenuated Total Reflection
Infrared Spectroscopy 420
10.1.4. Fundamentals of Surface-Enhanced
Infrared Spectroscopy 421
10.2. Sample Preparation for SERS 423
10.2.1. Electrochemical Techniques 423
10.2.2. Vapor Deposition and Chemical
Preparation Techniques 424
10.2.3. Colloidal Sol Techniques 425
10.2.4. Nanoparticle Arrays and Gratings 427
10.3. Sample Preparation for SEIRA 431
10.4. Potential Applications 433
References 436
INDEX 439

Warmly well come to the box Hoahoc, Master Huy (nickname Concay).
Tui đã đọc khá nhiều bài viết của bác bên box CNSH, kiếm được một người nhiệt tình như bác quả là hiếm có.
Hi vọng bác thấy bên box này có nhiều cái làm bác thoải mái
Warmly well come to the box Hoahoc

Em cũng rất thèm mấy quyển sách của bác, có điều bác cho em hỏi, sách bình thường hay điện tử. Mà sách ngoại giá có ngoại không?!
Cám ơn bác!

Nó là ebook, nhưng chúng tôi in với giá 500đồng/2 mặt. Giá cực kỳ nội.

Vậy phương thức thế nào bác nói đi?! Liệu bác có lấy được sách theo đặt hàng không?

Nếu bạn ở SG thì đến thẳng 201 Lương Nhữ Học Quận 5. Con nếu ở HN thì chỉ có cách là chuyển khoản hoặc gửi tiền bằng đường bưu điện.
Sách hóa học ebook thì vô tận, mà khả năng HuyNguyễn thì có hạn, nên không thể bừa bãi bảo OK mọi thứ; nhưng xin bạn cứ nói tên sách, NXB, nếu trong tầm của HN, chúng tôi sẽ cố gắng đáp ứng.
Ngoài sách, chúng tôi còn có thể đáp ứng các bài bào fulltext (dĩ nhiên nếu nó có bản online) theo đơn đặt hàng.
Mong chờ thư bạn

Anh gì ơi, thế anh ó cung cấp sách Hoá bằng tiếng Việt không ạ?
Tiện đây em cũng xin hỏi có anh chị nào có cuốn (sách thật không phải sách phô tô):
[

b]CƠ SỞ HOÁ HỌC HỮU CƠ (tập 1 và 2)​

của các thầy Phan tống Sơn - Trần quốc Sơn - Đặng Như Tại [/b] mà không dùng, muốn chuyển nhượng lại thì hãy liên lạc với em qua địa chỉ Yahoo: ruri_bee.
Thế nhé, em đợi tin mọi người !
Được dioxyl sửa chữa / chuyển vào 09:09 ngày 19/04/2004

O khong ban a, chung toi chi cung cap sach, bai bao HOA HOC bang tieng ANH thoi.

Mục lục sách mới cập nhật của HUY NGUYỄN.
Bạn nào có nhu cầu xin liên hệ theo địa chỉ 201 Lương Nhữ Học, p11, Q5 tp HCM.
email: phongtailieu@huynguyenltd.com
phone 08-9509938, 8574867
hotline: 0918190535 (Ngọc Hiệp)
0903030728 (Minh Lý)

ADVANCED THERMODYNAMICS FOR ENGINEERS
by Winterbone, Desmond E
Elsevier 1997 370
AGRICULTURAL CHEMICALS AND THE ENVIRONMENT
E***ed by Hester R.E.; Harrison, R.M.
Royal Society of Chemistry 1996 140
CHEMICAL PROCESS AND DESIGN HANDBOOK
by Speight, James G.
McGraw-Hill 2002 575
CHEMICAL PROCESS EQUIPMENT - SELECTION AND DESIGN
by Walas, S.M.
Elsevier 1990 755
CHEMICAL REACTOR DESIGN, OPTIMIZATION, AND SCALEUP
by Nauman, E. Bruce
McGraw-Hill 2002 624
CHEMISTRY IN THE MARINE ENVIRONMENT
E***ed by Hester R.E.; Harrison, R.M.
Royal Society of Chemistry 2000 112
CHEMISTRY OF THE ELEMENTS (2ND E***ION)
by Greenwood, N.N.; Earnshaw, A.
Elsevier 1997 1341
DESIGN OF DISTILLATION COLUMN CONTROL SYSTEMS
by Buckley, Page S.; Luyben, William L.; Shunta, Joseph P.
Elsevier 1985 532
ELECTROCHEMISTRY OF SEMICONDUCTORS AND ELECTRONICS - PROCESSES AND DEVICES
E***ed by McHardy, John; Ludwig, Frank
William Andrew Publishing/Noyes 1992 359
FUNDAMENTALS OF DAIRY CHEMISTRY (3RD E***ION)
E***ed by Wong, Noble P.; Jenness, Robert; Keeney, Mark; Marth, Elmer H.
Kluwer Academic Publishers 1999 779
HANDBOOK OF CHEMICAL PROCESSING EQUIPMENT
by Cheremisinoff, Nicholas P.
Elsevier 2000 435
INSTRUMENTATION FOR FLUID-PARTICLE FLOW
E***ed by Soo, Shao Lee
William Andrew Publishing/Noyes 1999 412
MATERIALS SELECTION DESKBOOK
by Cheremisinoff, Nicholas P.
William Andrew Publishing/Noyes 1996 191
NALCO WATER HANDBOOK (2ND E***ION)
E***ed by Kemmer, F.N.
McGraw-Hill 1988 1120
ONDEONALCO FUEL FIELD MANUAL (REVISED E***ION)
by Peyton, K.B.
McGraw-Hill 2002 380
PARTICLE DEPOSITION AND AGGREGATION - MEASUREMENT, MODELLING AND SIMULATION
by Elimelech, M.; Gregory, J.; Jia, X.; Williams. R.A.
Elsevier 1995 441
PIPING HANDBOOK (7TH E***ION)
E***ed by Nayyar, Mohinder L.
McGraw-Hill 2000 2256
PROCESS ENGINEERING EQUIPMENT HANDBOOK
by Soares, Claire
McGraw-Hill 2002 874
PUMP HANDBOOK (3RD EDTION)
E***ed by Karassik, Igor J.; Messina, Joseph P.; Cooper, Paul; Heald, Charles C.
McGraw-Hill 2001 1765
PURIFICATION OF LABORATORY CHEMICALS (4TH E***ION)
by Armarego, W.L.F.; Perrin, D.D.
Elsevier 1996 529
PURIFICATION OF LABORATORY CHEMICALS (5TH E***ION)
by Armarego, Wilfred, L.F.; Chai, Christina, L.L.
Elsevier 2003 609
RESERVOIR ENGINEERING HANDBOOK (2ND E***ION)
by Ahmed, T.
Elsevier 2001 1208
SCIENCE AND ENGINEERING OF DROPLETS - FUNDAMENTALS AND APPLICATIONS
by Liu, Huimin
William Andrew Publishing/Noyes 2000 527
SMITHSONIAN PHYSICAL TABLES (9TH REVISED E***ION)
by Forsythe, W.E.
Knovel 2003 837
THERMOCHEMISTRY OF THE CHEMICAL SUBSTANCES
by Bichowsky, F.R.; Rossini, F.D.
Knovel 2003 460
TUNGSTEN - PROPERTIES, CHEMISTRY, TECHNOLOGY OF THE ELEMENT, ALLOYS, AND CHEMICAL COMPOUNDS
by Lassner, Erik; Schubert, Wolf-Dieter
Kluwer Academic Publishers 1999 422