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Blood samples were centrifuged for 10 min at 18 700 g and plasma was pipetted off.
Pipetted 1:20 hemolysate of plasma was used for determination of the malondialdehyde
level, oxidative modification of plasma proteins, total antioxidant activity of
blood and catalase activity.
Lipid peroxidation was determined by measuring the concentration of thiobarbituric
acid reactive substances (TBARS) concentration (micromole per liter of blood), it
was done as described by Uchiyama and Mihara (1978). Carbonyl groups formed
from oxidation with 2.4-dinitrophenyl hydrazine (DNPH) were estimated using the
methods by Levine et al. (1990) with modifications (Dubinina et al. 2000). Estimation
of derivatives of 2.4-dinitrophenyl hydrazones in blood may serve as a pattern
of oxidative modification of proteins during oxidative stress in cells. The levels of
total antioxidant activity in the blood were estimated following the method with Fe​

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2+​

and ascorbate-dependent Tween-80 oxidation using method by Halaktionova et al.
(1998). Superoxide dismutase (SOD; E.C. 1.15.1.1) activity was measured by the
quercetine method after suitable dilution following the method by Kostyuk et al.​

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(1990). The catalase activity was evaluated using the hydrogen peroxide breakdown
method (Korolyuk et al. 1988). Glutathione reductase (GR; E.C. 1.6.4.2) activity
was determined by reduced NADPH and oxidized glutathione (GSSG) substrates in
the 1:20 hemolysate measured according to the method described by Glatzle et al.
(1974). Glutathione peroxidase (EC 1.11.1.9) activity was measured following the​

method of Moin (1986).